Toxin A (enterotoxin) from Clostridium difficile in antibiotic-associated colitis.

severe, interstitial pneumonitis of undetermined cause. The remaining animals were anaesthetised, systemically perfused with buffered solutions of paraformaldehyde followed by glutaraldehyde, and tissue prepared for examination in 1 m epoxy cross-sections stained with toluidine-blue. Examination of cortex, cerebellum, spinal cord, sciatic-nerve complex, and gastrocnemius muscle revealed no toxin-related pathological changes. Thus authentic samples of contaminated oil did not induce neuromuscular disease in a species that is sensitive to most neurotoxins. The relative doses were much larger than the amounts ingested by victims of the Spanish oil syndrome and the exposure